Luminescence Image Analyzer
  Cytiva  A :ImageQuant 800  B :ImageQuant 800 Fluor
       Installation date: March, 2022
       A :Restricted to “chemiluminescence”
       B : For “chemiluminescence”, “fluorescence”, “gel analysis”, and “OD (Optical Density Measurements)”
  • Common to “A” and “B”
  •   It is a CCD-based imager detecting both chemiluminescence and visible spectrum.
  •   Enables easy to perform high sensitivity detection of western blotting without the need of a darkroom.
  •   Pre-Capture :
  •     automatically calculates the exposure time of the region of interest in the pre-captured image.
  •   SNOW(Signal Noise Optimization Watch :
  •     reduces noise and avoids saturation of the image by multiple exposures and merging them into a single signal. Both weak and strong bands can be captured without saturation. (e.g. phosphorylation bands, close bands)
  •   Both chemiluminescence sample image and colorimetric marker image can be captured and automatically overlaid.
  •    Image formats: 16bit gray scale TIF (Raw data) and JPEG (finalized image if edited)
  •    ImageQuant TL (analysis software) is available.
  • Restricted to “B”
  •   Fluorescent imaging (A maximum of three dyes can be analyzed at one time by “Auto” mode and “manual” mode.)
  •   Chemiluminescence imaging and fluorescence imaging can be combined. (e.g. First, capture the target protein band with chemiluminescence and then, capture the house keeping band with fluorescence, and merge them automatically.)
  •   Gel documentation such as capturing images of CBB stained gels (White Epi light source)
  •   OD (Optical Density) measurements (White Trans light source)

  •  You must receive a training session.
  •    “A” should be used for chemiluminescense image capture.
  •   Neither “A” nor “B” can be used for EtBr detection.
  •   There are restrictions on time of use: 1 hour
  •   When using SNOW, which takes longer, use “B” preferentially. (Please consult us when you make a reservation.)

  About training session
  •   We conduct training sessions as needed. Please contact us in advance to adjust the schedule.
  •   The training session takes approximately 1 hour.
  •     Please contact us after you have decided when you will start using the equipment.
  •     There is no need to bring a sample, but if you wish to bring one please let us know.

  Specifications (Restricted to “B”)
      Filter and LED
      Band-pass filter allows light with particular wavelength to pass through.

Copyright(C)The Research Support Center, Research Center for Human Disease Modeling,
Kyushu University Graduate School of Medical Sciences. All Rights Reserved.